Removal of labile glycosylated hemoglobin to correct for fluctuations in hemoglobin A1 measurement by column chromatography.
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چکیده
منابع مشابه
Labile glycosylated hemoglobin contributes to hemoglobin A1 as measured by liquid chromatography or electrophoresis.
The utility of glycosylated hemoglobin (HbA1) measurement as an index of chronic control in diabetes can be adversely affected by interference from a labile glycosylated fraction that changes rapidly with acute changes in blood glucose concentration. I used a "high-pressure" liquid-chromatographic assay and a newly developed electrophoretic assay to quantitate the contribution of this labile fr...
متن کاملRapid method for eliminating labile glycosylated hemoglobin from the assay for hemoglobin A1.
The irreversible formation of stable glycosylated hemoglobin proceeds through a labile intermediate that is indistinguishable, by most methods, from the stable glycosylated product. The inclusion of the labile intermediate, which changes acutely with acute blood glucose changes, detracts from the utility of the assay as an index of chronic glucose concentration. We have developed a rapid, relia...
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The anode Is at the left, the origin at the rlt. Above Is a control consIstIng of HbA, F. S. and A2.Below Is the concentrated column eluate. Note the fast hemoglobIn band at the far left and carbonIc anhy-&ase B near the origIn atthe rIght In the pattern for the column eluate 1250 CLINICAL CHEMISTRY. dure is applicable also to other situations , e.g.,to the measurements of specific radioactivit...
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We present chromatographic conditions for separating and measuring fetal hemoglobin on a 5-cm column of Sephadex CM-50. A distinct fetal band is evident, even when present at less than 1% of total hemoglobin. Precision (CV) of better than 5% is achievable.
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Haptoglobin (Hp) is an acute-phase protein; its plasma levels increase consistently in response to infection and inflammation. The concentration of human plasma Hp is ranged between 1 and 1.5 mg/ml. Similar to blood type, individual human Hp is classified as Hp 1-1, 2-1, or 2-2. The structural and functional analysis of the Hp, however, has not been studied in detail due to its difficult isolat...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1982
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/28.9.1993